To solve the problem that current computer serial communication only with USB interface can not satisfy with the old type equipments or MCU to communicate with RS232, two kinds of RS232/USB circuit were designed.CH341A and MAX223 integrated circuit chips were used to create a standard 9-line RS232/USB universal interface convertor without programme. CH341A and PIC16F877A chips were adopted to build the USB communication circuit between computers and MCU. The software follows RS232 communication protocol, and the hardware converts electrical levels. Actual practices indicate that the two manufactures are compatible with serial application program of Windows operation system completely,and get avoid of handshake lost.