lifefitascii_single.m

算荧光衰减时间或者寿命的软件.matlab语言编写.

% Program for the Calculation of Fluorescence Lifetimes
% Dr. Joshua B. Edel


%%%%%%%%%%%%%%%% Initialize
clear all;
close all;
warning off;
set(0, 'defaultfigurecolor', [ 1, 1, 1 ]);

%%%%%%%%%%%%%%%%%%%%% Parameters %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
file = 'C:\Documents and Settings\Meller\Desktop\J-Lfe ASCII\sample.txt';
InstCurveNum = 1; % Instrument response column number
DataCurveNum = 3; % decay column number
TimePerChan = 0.0061035157; % time per channel in ns
Amp = [0.01   0.02      0.01]; % initial amplitude guess
Life = [1   2      5]; % initial lifetime guess
components = 2; % max of 3 components


%%%%%%%%%%%%%%%%%%%% Boundary conditions
[StartTime, EndTime,lb,ub] = Boundary(file,InstCurveNum,DataCurveNum);

%%%%%%%%%%%%%%%%%%%% Fitting routine
[ExportData,FittedDecays] = MainRoutine(file,StartTime,EndTime,Amp,Life,InstCurveNum,DataCurveNum,components,lb,ub,TimePerChan);


%%%%%%%%%%%%%%%%%%%% Instructions
% 1) hello
% 
% 2) To deconvolve IRF from decay a tab delim text file is needed with
% columns containing Instrument Response and Decay. a sample file is given
% called sample.txt
% 
% 3) All columns must have the same length.
% 
% 4) Adjust inital lifetime, amplitude,boundary conditions, channels, etc....
% Boundary conditions can be changed in the "Boundary.m" file

% 5) The inital Amp and Life vectors should have length of 3 hence for 1
% component fit only change the 1st value etc....
% 
% 6) Run program. A figure will be displayed containing the IRF and decay.
% select the area of interest by drawing a box with your mouse around the area that you
% want analyzed

% 7) Analysis and fit output is summarized in text files in the same folder as the decay file
% 
% 8) For further help please contact josh at [e-mail]
% 
% 9) This program is freeware however, if this program is used towards a publication please mention that J-life was used in an appropriate section of the paper.
% 
% 10) Enjoy the program.
% 
% 
% Dr. Joshua B. Edel
% Rowland Institute at Harvard
% Harvard University
% 100 Edwin H. Land Blvd.
% Cambridge, MA 02142
% 
% Tel: [phone]
% Fax: [phone]