form2.cs

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using System;
using System.Collections.Generic;
using System.ComponentModel;
using System.Data;
using System.Drawing;
using System.Text;
using System.Windows.Forms;

namespace PictureDemo
{
    public partial class Form2 : Form
    {
        public Form2()
        {
            InitializeComponent();
        }

        private void button2_Click(object sender, EventArgs e)
        {
            this.timer1.Stop();
        }
        
        private void button1_Click(object sender, EventArgs e)
        {
            this.timer1.Start();

        }
         int i = 1;
        private void timer1_Tick(object sender, EventArgs e)
        {
            if (i < 46)
            {
                i++;
                string path = @"img\" + i + ".gif";
                this.pictureBox1.Image = Image.FromFile(path);
            }
            else
            {
                i = 1;
            }
        }
    }
}