eprimer3.output

emboss的linux版本的源代码

<a name="output.1"></a>
<h3>Output files for usage example </h3>
<p><h3>File: hsfau.eprimer3</h3>
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# EPRIMER3 RESULTS FOR HSFAU1

# FORWARD PRIMER STATISTICS:
# considered 16422
# GC content failed 156
# low tm 3616
# high tm 9306
# high any compl 9
# high end compl 26
# long poly-x seq 45
#high 3' stability 304
# ok 2960

# REVERSE PRIMER STATISTICS:
# considered 16330
# GC content failed 161
# low tm 3580
# high tm 9309
# high any compl 2
# high end compl 9
# long poly-x seq 66
#high 3' stability 301
# ok 2902

# PRIMER PAIR STATISTICS:
# considered 2652
# unacceptable product size 2444
# high any compl 2
# high end compl 20
# ok 186

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER     801   20  59.72  60.00  CCCAGGAGCTACACACCTTC

     REVERSE PRIMER     983   20  59.81  55.00  GGAGCACGACTTGATCTTCC


   2 PRODUCT SIZE: 203
     FORWARD PRIMER    1810   20  59.43  55.00  GAAGAAGACAGGTCGGGCTA

     REVERSE PRIMER    1993   20  59.99  60.00  GAAGCACACCTCTCCCTGAG


   3 PRODUCT SIZE: 204
     FORWARD PRIMER    1810   20  59.43  55.00  GAAGAAGACAGGTCGGGCTA

     REVERSE PRIMER    1994   20  59.99  55.00  AGAAGCACACCTCTCCCTGA


   4 PRODUCT SIZE: 196
     FORWARD PRIMER    1266   20  59.43  60.00  GGGAGACTGAGCCAGAGTGT

     REVERSE PRIMER    1442   20  60.06  50.00  GCGCCCAATAGGTAATGAGA


   5 PRODUCT SIZE: 205
     FORWARD PRIMER     801   20  59.72  60.00  CCCAGGAGCTACACACCTTC

     REVERSE PRIMER     986   20  60.41  55.00  CCAGGAGCACGACTTGATCT



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