📄 rebaseextract.output
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<a name="output.1"></a><h3>Output files for usage example </h3><p><h3>Directory: REBASE</h3><p>This directory contains output files, for example embossre.enz embossre.equ embossre.ref and embossre.sup.<p><h3>File: REBASE/embossre.enz</h3><table width="90%"><tr><td bgcolor="#CCFFCC"><pre># REBASE enzyme patterns for EMBOSS## Format:# name<ws>pattern<ws>len<ws>ncuts<ws>blunt<ws>c1<ws>c2<ws>c3<ws>c4## Where:# name = name of enzyme# pattern = recognition site# len = length of pattern# ncuts = number of cuts made by enzyme# Zero represents unknown# blunt = true if blunt end cut, false if sticky# c1 = First 5' cut# c2 = First 3' cut# c3 = Second 5' cut# c4 = Second 3' cut## Examples:# AAC^TGG -> 6 2 1 3 3 0 0# A^ACTGG -> 6 2 0 1 5 0 0# AACTGG -> 6 0 0 0 0 0 0# AACTGG(-5/-1) -> 6 2 0 1 5 0 0# (8/13)GACNNNNNNTCA(12/7) -> 12 4 0 -9 -14 24 19## i.e. cuts are always to the right of the given# residue and sequences are always with reference to# the 5' strand.# Sequences are numbered ... -3 -2 -1 1 2 3 ... with# the first residue of the pattern at base number 1.#AaeI ggatcc 6 0 0 0 0 0 0AciI CCGC 4 2 0 1 3 0 0AclI AACGTT 6 2 0 2 4 0 0BamHI GGATCC 6 2 0 1 5 0 0BceAI ACGGC 5 2 0 17 19 0 0Bsc4I CCNNNNNNNGG 11 2 0 7 4 0 0Bse1I ACTGG 5 2 0 6 4 0 0BseYI CCCAGC 6 2 0 1 5 0 0BshI GGCC 4 2 1 2 2 0 0BsiSI CCGG 4 2 0 1 3 0 0BsiYI CCNNNNNNNGG 11 2 0 7 4 0 0BssKI CCNGG 5 2 0 -1 5 0 0BsrI ACTGG 5 2 0 6 4 0 0Bsu6I CTCTTC 6 2 0 7 10 0 0ClaI ATCGAT 6 2 0 2 4 0 0EcoRI GAATTC 6 2 0 1 5 0 0EcoRII CCWGG 5 2 0 -1 5 0 0HaeIII GGCC 4 2 1 2 2 0 0HhaI GCGC 4 2 0 3 1 0 0Hin4I GAYNNNNNVTC 11 4 0 -9 -14 24 19Hin6I GCGC 4 2 0 1 3 0 0HinP1I GCGC 4 2 0 1 3 0 0HindI cac 3 0 0 0 0 0 0HindII GTYRAC 6 2 1 3 3 0 0HindIII AAGCTT 6 2 0 1 5 0 0HpaII CCGG 4 2 0 1 3 0 0HpyCH4IV ACGT 4 2 0 1 3 0 0HspAI GCGC 4 2 0 1 3 0 0KpnI GGTACC 6 2 0 5 1 0 0Ksp632I CTCTTC 6 2 0 7 10 0 0MaeII ACGT 4 2 0 1 3 0 0NotI GCGGCCGC 8 2 0 2 6 0 0TaqI TCGA 4 2 0 1 3 0 0</pre></td></tr></table><p><p><h3>File: REBASE/embossre.equ</h3><table width="90%"><tr><td bgcolor="#CCFFCC"><pre>Bsc4I BsiYIBse1I BsrIBshI HaeIIIBsiSI HpaIIBsu6I Ksp632IHpyCH4IV MaeII</pre></td></tr></table><p><p><h3>File: REBASE/embossre.ref</h3><table width="90%"><tr><td bgcolor="#CCFFCC"><pre># REBASE enzyme information for EMBOSS## Format:# Line 1: Name of Enzyme# Line 2: Organism# Line 3: Isoschizomers# Line 4: Methylation# Line 5: Source# Line 6: Suppliers# Line 7: Number of following references# Lines 8..n: References# // (end of entry marker)#AaeIAcetobacter aceti sub. liquefaciensBamHI,AacI,AcaII,AccEBI,AinII,AliI,Ali12257I,Ali12258I,ApaCI,AsiI,AspTII,Atu1II,BamFI,BamKI,BamNI,Bca1259I,Bce751I,Bco10278I,BnaI,BsaDI,Bsp30I,Bsp46I,Bsp90II,Bsp98I,Bsp130I,Bsp131I,Bsp144I,Bsp4009I,BspAAIII,BstI,Bst1126I,Bst2464I,Bst2902I,BstQI,Bsu90I,Bsu8565I,Bsu8646I,BsuB519I,BsuB763I,CelI,DdsI,GdoI,GinI,GoxI,GseIII,MleI,Mlu23I,NasBI,Nsp29132II,NspSAIV,OkrAI,Pac1110I,Pae177I,Pfl8I,Psp56I,RhsI,Rlu4I,RspLKII,SolI,SpvI,SurI,Uba19I,Uba31I,Uba38I,Uba51I,Uba88I,Uba1098I,Uba1163I,Uba1167I,Uba1172I,Uba1173I,Uba1205I,Uba1224I,Uba1242I,Uba1250I,Uba1258I,Uba1297I,Uba1302I,Uba1324I,Uba1325I,Uba1334I,Uba1339I,Uba1346I,Uba1383I,Uba1398I,Uba1402I,Uba1414IM. Van Montagu1Seurinck, J., van Montagu, M., Unpublished observations.//AciIArthrobacter citreus?(5),-2(5)NEB 577N2Lunnen, K.D., Heiter, D., Wilson, G.G., Unpublished observations.Polisson, C., Morgan, R.D., (1990) Nucleic Acids Res., vol. 18, pp. 5911.//AclIAcinetobacter calcoaceticus M4Psp1406I3(5)S.K. DegtyarevIN2Degtyarev, S.K., Abdurashitov, M.A., Kolyhalov, A.A., Rechkunova, N.I., (1992) Nucleic Acids Res., vol. 20, pp. 3787.Lunnen, K.D., Wilson, G.G., Unpublished observations.//BamHIBacillus amyloliquefaciens HAacI,AaeI,AcaII,AccEBI,AinII,AliI,Ali12257I,Ali12258I,ApaCI,AsiI,AspTII,Atu1II,BamFI,BamKI,BamNI,Bca1259I,Bce751I,Bco10278I,BnaI,BsaDI,Bsp30I,Bsp46I,Bsp90II,Bsp98I,Bsp130I,Bsp131I,Bsp144I,Bsp4009I,BspAAIII,BstI,Bst1126I,Bst2464I,Bst2902I,BstQI,Bsu90I,Bsu8565I,Bsu8646I,BsuB519I,BsuB763I,CelI,DdsI,GdoI,GinI,GoxI,GseIII,MleI,Mlu23I,NasBI,Nsp29132II,NspSAIV,OkrAI,Pac1110I,Pae177I,Pfl8I,Psp56I,RhsI,Rlu4I,RspLKII,SolI,SpvI,SurI,Uba19I,Uba31I,Uba38I,Uba51I,Uba88I,Uba1098I,Uba1163I,Uba1167I,Uba1172I,Uba1173I,Uba1205I,Uba1224I,Uba1242I,Uba1250I,Uba1258I,Uba1297I,Uba1302I,Uba1324I,Uba1325I,Uba1334I,Uba1339I,Uba1346I,Uba1383I,Uba1398I,Uba1402I,Uba1414I5(4)ATCC 49763ABCDEFGHIJKLMNOQRSTUV10Brooks, J.E., Howard, K.A., US Patent Office, 1994.<font color=red> [Part of this file has been deleted for brevity]</font>ATCC 49790ABCDEFGHIJKLMNOQRSTU3Kiss, A., Finta, C., Venetianer, P., (1991) Nucleic Acids Res., vol. 19, pp. 3460.Smith, D.I., Blattner, F.R., Davies, J., (1976) Nucleic Acids Res., vol. 3, pp. 343-353.Tomassini, J., Roychoudhury, R., Wu, R., Roberts, R.J., (1978) Nucleic Acids Res., vol. 5, pp. 4055-4064.//Ksp632IKluyvera species 632Bco5I,Bco116I,BcoKI,BcoSI,BcrAI,BseZI,BsrEI,Bst6I,Bst158I,Bsu6I,Eam1104I,EarI,TdeII,Uba1192I,Uba1276I,VpaKutEI,VpaKutFI,VpaO5IDSM 4196M2Bolton, B.J., Schmitz, G.G., Jarsch, M., Comer, M.J., Kessler, C., (1988) Gene, vol. 66, pp. 31-43.Tsukahara, S., Yamakawa, H., Takai, K., Takaku, H., (1994) Nucleosides & Nucleotides, vol. 13, pp. 1617-1626.//MaeIIMethanococcus aeolicus PL-15/HHpyCH4IV,HpyF13III,HpyF35II,HpyF74II,TaiI,TscI,Tsp49I,TspIDSI,TspWAM8AI,TtmIK.O. StetterM1Schmid, K., Thomm, M., Laminet, A., Laue, F.G., Kessler, C., Stetter, K.O., Schmitt, R., (1984) Nucleic Acids Res., vol. 12, pp. 2619-2628.//NotINocardia otitidis-caviarumCciNI,CspBI,MchAI?(4)ATCC 14630ABCDEFGHJKLMNOQRSTU4Borsetti, R., Wise, D., Qiang, B.-Q., Schildkraut, I., Unpublished observations.Morgan, R.D., Unpublished observations.Morgan, R.D., Benner, J.S., Claus, T.E., US Patent Office, 1994.Qiang, B.-Q., Schildkraut, I., (1987) Methods Enzymol., vol. 155, pp. 15-21.//TaqIThermus aquaticus YTICviSIII,EsaBC3I,HpyV,Hpy26II,HpyF14III,HpyF16I,HpyF23I,HpyF24I,HpyF26III,HpyF30I,HpyF35I,HpyF40II,HpyF42IV,HpyF45I,HpyF49I,HpyF52I,HpyF59III,HpyF62II,HpyF64I,HpyF65II,HpyF66IV,HpyF71I,HpyF73II,HpyJP26II,PpaAII,Taq20I,Tbr51I,TfiA3I,TfiTok4A2I,TfiTok6A1I,TflI,Tsc4aI,Tsp32I,Tsp32II,Tsp358I,Tsp505I,Tsp510I,TspAK13D21I,TspAK16D24I,TspNI,TspVi4AI,TspVil3I,Tth24I,TthHB8I,TthRQI4(6)J.I. HarrisABCDEFGIJLMNOQRSTU5Anton, B.P., Brooks, J.E., Unpublished observations.Fomenkov, A., Xiao, J.-P., Dila, D., Raleigh, E., Xu, S.-Y., (1994) Nucleic Acids Res., vol. 22, pp. 2399-2403.McClelland, M., (1981) Nucleic Acids Res., vol. 9, pp. 6795-6804.Sato, S., Hutchison, C.A. III, Harris, J.I., (1977) Proc. Natl. Acad. Sci. U. S. A., vol. 74, pp. 542-546.Zebala, J.A., (1993) Diss. Abstr., vol. 54, pp. 1394-1398.//</pre></td></tr></table><p><p><h3>File: REBASE/embossre.sup</h3><table width="90%"><tr><td bgcolor="#CCFFCC"><pre># REBASE Supplier information for EMBOSS## Format:# Code of Supplier<ws>Supplier name#A Amersham Pharmacia Biotech (1/01)B Life Technologies Inc. (1/98)C Minotech, Molecular Biology Products (12/00)D HYBAID GmbH (12/00)E Stratagene (11/00)F Fermentas AB (5/01)G Q-BIOgene (1/01)H American Allied Biochemical, Inc. (10/98)I SibEnzyme Ltd. (1/01)J Nippon Gene Co., Ltd. (6/00)K Takara Shuzo Co. Ltd. (2/01)L Transgenomic Ltd. (1/01)M Roche Molecular Biochemicals (1/01)N New England BioLabs (12/00)O Toyobo Biochemicals (11/98)P Megabase Research Products (5/99)Q CHIMERx (10/97)R Promega Corporation (6/99)S Sigma Chemical Corporation (11/98)T Advanced Biotechnologies Ltd. (3/98)U Bangalore Genei (2/01)V MRC-Holland (3/01)</pre></td></tr></table><p>
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