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Standard (Mandatory) qualifiers (* if not always prompted): [-sequence] seqall Nucleotide sequence(s) filename and optional format, or reference (input USA) -windowsize integer [20] The values of melting point and other thermodynamic properties of the sequence are determined by taking a short length of sequence known as a window and determining the properties of the sequence in that window. The window is incrementally moved along the sequence with the properties being calculated at each new position. (Integer from 1 to 100) -shiftincrement integer [1] This is the amount by which the window is moved at each increment in order to find the melting point and other properties along the sequence. (Integer 1 or more) -dnaconc float [50.] Enter DNA concentration (nM) (Number from 1.000 to 100000.000) -saltconc float [50.] Enter salt concentration (mM) (Number from 1.000 to 1000.000)* -mintemp float [55.] Enter a minimum value for the temperature scale (y-axis) of the plot. (Number from 0.000 to 150.000)* -graph xygraph [$EMBOSS_GRAPHICS value, or x11] Graph type (ps, hpgl, hp7470, hp7580, meta, cps, x11, tekt, tek, none, data, xterm, png)* -outfile report [*.dan] If a plot is not being produced then data on the melting point etc. in each window along the sequence is output to the file. Additional (Optional) qualifiers (* if not always prompted): -product toggle This prompts for percent formamide, percent of mismatches allowed and product length.* -formamide float [0.] This specifies the percent formamide to be used in calculations (it is ignored unless -product is used). (Number from 0.000 to 100.000)* -mismatch float [0.] This specifies the percent mismatch to be used in calculations (it is ignored unless -product is used). (Number from 0.000 to 100.000)* -prodlen integer [Window size (20)] This specifies the product length to be used in calculations (it is ignored unless -product is used). (Any integer value) -thermo toggle Output the DeltaG, DeltaH and DeltaS values of the sequence windows to the output data file.* -temperature float [25.] If -thermo has been specified then this specifies the temperature at which to calculate the DeltaG, DeltaH and DeltaS values. (Number from 0.000 to 100.000) Advanced (Unprompted) qualifiers: -rna boolean This specifies that the sequence is an RNA sequence and not a DNA sequence. -plot toggle If this is not specified then the file of output data is produced, else a plot of the melting point along the sequence is produced. Associated qualifiers: "-sequence" associated qualifiers -sbegin1 integer Start of each sequence to be used -send1 integer End of each sequence to be used -sreverse1 boolean Reverse (if DNA) -sask1 boolean Ask for begin/end/reverse -snucleotide1 boolean Sequence is nucleotide -sprotein1 boolean Sequence is protein -slower1 boolean Make lower case -supper1 boolean Make upper case -sformat1 string Input sequence format -sdbname1 string Database name -sid1 string Entryname -ufo1 string UFO features -fformat1 string Features format -fopenfile1 string Features file name "-graph" associated qualifiers -gprompt boolean Graph prompting -gdesc string Graph description -gtitle string Graph title -gsubtitle string Graph subtitle -gxtitle string Graph x axis title -gytitle string Graph y axis title -goutfile string Output file for non interactive displays -gdirectory string Output directory "-outfile" associated qualifiers -rformat string Report format -rname string Base file name -rextension string File name extension -rdirectory string Output directory -raccshow boolean Show accession number in the report -rdesshow boolean Show description in the report -rscoreshow boolean Show the score in the report -rusashow boolean Show the full USA in the report -rmaxall integer Maximum total hits to report -rmaxseq integer Maximum hits to report for one sequence General qualifiers: -auto boolean Turn off prompts -stdout boolean Write standard output -filter boolean Read standard input, write standard output -options boolean Prompt for standard and additional values -debug boolean Write debug output to program.dbg -verbose boolean Report some/full command line options -help boolean Report command line options. More information on associated and general qualifiers can be found with -help -verbose -warning boolean Report warnings -error boolean Report errors -fatal boolean Report fatal errors -die boolean Report dying program messages
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