📄 rebaseextract.txt
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# c3 = Second 5' cut# c4 = Second 3' cut## Examples:# AAC^TGG -> 6 2 1 3 3 0 0# A^ACTGG -> 6 2 0 1 5 0 0# AACTGG -> 6 0 0 0 0 0 0# AACTGG(-5/-1) -> 6 2 0 1 5 0 0# (8/13)GACNNNNNNTCA(12/7) -> 12 4 0 -9 -14 24 19## i.e. cuts are always to the right of the given# residue and sequences are always with reference to# the 5' strand.# Sequences are numbered ... -3 -2 -1 1 2 3 ... with# the first residue of the pattern at base number 1.#AaeI ggatcc 6 0 0 0 0 0 0AciI CCGC 4 2 0 1 3 0 0AclI AACGTT 6 2 0 2 4 0 0BamHI GGATCC 6 2 0 1 5 0 0BceAI ACGGC 5 2 0 17 19 0 0Bsc4I CCNNNNNNNGG 11 2 0 7 4 0 0Bse1I ACTGG 5 2 0 6 4 0 0BseYI CCCAGC 6 2 0 1 5 0 0BshI GGCC 4 2 1 2 2 0 0BsiSI CCGG 4 2 0 1 3 0 0BsiYI CCNNNNNNNGG 11 2 0 7 4 0 0BssKI CCNGG 5 2 0 -1 5 0 0BsrI ACTGG 5 2 0 6 4 0 0Bsu6I CTCTTC 6 2 0 7 10 0 0ClaI ATCGAT 6 2 0 2 4 0 0EcoRI GAATTC 6 2 0 1 5 0 0EcoRII CCWGG 5 2 0 -1 5 0 0HaeIII GGCC 4 2 1 2 2 0 0HhaI GCGC 4 2 0 3 1 0 0Hin4I GAYNNNNNVTC 11 4 0 -9 -14 24 19Hin6I GCGC 4 2 0 1 3 0 0HinP1I GCGC 4 2 0 1 3 0 0HindI cac 3 0 0 0 0 0 0HindII GTYRAC 6 2 1 3 3 0 0HindIII AAGCTT 6 2 0 1 5 0 0HpaII CCGG 4 2 0 1 3 0 0HpyCH4IV ACGT 4 2 0 1 3 0 0HspAI GCGC 4 2 0 1 3 0 0KpnI GGTACC 6 2 0 5 1 0 0Ksp632I CTCTTC 6 2 0 7 10 0 0MaeII ACGT 4 2 0 1 3 0 0NotI GCGGCCGC 8 2 0 2 6 0 0TaqI TCGA 4 2 0 1 3 0 0 File: REBASE/embossre.equBsc4I BsiYIBse1I BsrIBshI HaeIIIBsiSI HpaIIBsu6I Ksp632IHpyCH4IV MaeII File: REBASE/embossre.ref# REBASE enzyme information for EMBOSS## Format:# Line 1: Name of Enzyme# Line 2: Organism# Line 3: Isoschizomers# Line 4: Methylation# Line 5: Source# Line 6: Suppliers# Line 7: Number of following references# Lines 8..n: References# // (end of entry marker)#AaeIAcetobacter aceti sub. liquefaciensBamHI,AacI,AcaII,AccEBI,AinII,AliI,Ali12257I,Ali12258I,ApaCI,AsiI,AspTII,Atu1II,BamFI,BamKI,BamNI,Bca1259I,Bce751I,Bco10278I,BnaI,BsaDI,Bsp30I,Bsp46I,Bsp90II,Bsp98I,Bsp130I,Bsp131I,Bsp144I,Bsp4009I,BspAAIII,BstI,Bst1126I,Bst2464I,Bst2902I,BstQI,Bsu90I,Bsu8565I,Bsu8646I,BsuB519I,BsuB763I,CelI,DdsI,GdoI,GinI,GoxI,GseIII,MleI,Mlu23I,NasBI,Nsp29132II,NspSAIV,OkrAI,Pac1110I,Pae177I,Pfl8I,Psp56I,RhsI,Rlu4I,RspLKII,SolI,SpvI,SurI,Uba19I,Uba31I,Uba38I,Uba51I,Uba88I,Uba1098I,Uba1163I,Uba1167I,Uba1172I,Uba1173I,Uba1205I,Uba1224I,Uba1242I,Uba1250I,Uba1258I,Uba1297I,Uba1302I,Uba1324I,Uba1325I,Uba1334I,Uba1339I,Uba1346I,Uba1383I,Uba1398I,Uba1402I,Uba1414IM. Van Montagu1Seurinck, J., van Montagu, M., Unpublished observations.//AciIArthrobacter citreus?(5),-2(5)NEB 577N2Lunnen, K.D., Heiter, D., Wilson, G.G., Unpublished observations.Polisson, C., Morgan, R.D., (1990) Nucleic Acids Res., vol. 18, pp. 5911.//AclIAcinetobacter calcoaceticus M4Psp1406I3(5)S.K. DegtyarevIN2Degtyarev, S.K., Abdurashitov, M.A., Kolyhalov, A.A., Rechkunova, N.I., (1992)Nucleic Acids Res., vol. 20, pp. 3787.Lunnen, K.D., Wilson, G.G., Unpublished observations.//BamHIBacillus amyloliquefaciens HAacI,AaeI,AcaII,AccEBI,AinII,AliI,Ali12257I,Ali12258I,ApaCI,AsiI,AspTII,Atu1II,BamFI,BamKI,BamNI,Bca1259I,Bce751I,Bco10278I,BnaI,BsaDI,Bsp30I,Bsp46I,Bsp90II,Bsp98I,Bsp130I,Bsp131I,Bsp144I,Bsp4009I,BspAAIII,BstI,Bst1126I,Bst2464I,Bst2902I,BstQI,Bsu90I,Bsu8565I,Bsu8646I,BsuB519I,BsuB763I,CelI,DdsI,GdoI,GinI,GoxI,GseIII,MleI,Mlu23I,NasBI,Nsp29132II,NspSAIV,OkrAI,Pac1110I,Pae177I,Pfl8I,Psp56I,RhsI,Rlu4I,RspLKII,SolI,SpvI,SurI,Uba19I,Uba31I,Uba38I,Uba51I,Uba88I,Uba1098I,Uba1163I,Uba1167I,Uba1172I,Uba1173I,Uba1205I,Uba1224I,Uba1242I,Uba1250I,Uba1258I,Uba1297I,Uba1302I,Uba1324I,Uba1325I,Uba1334I,Uba1339I,Uba1346I,Uba1383I,Uba1398I,Uba1402I,Uba1414I5(4)ATCC 49763ABCDEFGHIJKLMNOQRSTUV10Brooks, J.E., Howard, K.A., US Patent Office, 1994. [Part of this file has been deleted for brevity]ATCC 49790ABCDEFGHIJKLMNOQRSTU3Kiss, A., Finta, C., Venetianer, P., (1991) Nucleic Acids Res., vol. 19, pp. 3460.Smith, D.I., Blattner, F.R., Davies, J., (1976) Nucleic Acids Res., vol. 3, pp. 343-353.Tomassini, J., Roychoudhury, R., Wu, R., Roberts, R.J., (1978) Nucleic Acids Res., vol. 5, pp. 4055-4064.//Ksp632IKluyvera species 632Bco5I,Bco116I,BcoKI,BcoSI,BcrAI,BseZI,BsrEI,Bst6I,Bst158I,Bsu6I,Eam1104I,EarI,TdeII,Uba1192I,Uba1276I,VpaKutEI,VpaKutFI,VpaO5IDSM 4196M2Bolton, B.J., Schmitz, G.G., Jarsch, M., Comer, M.J., Kessler, C., (1988) Gene, vol. 66, pp. 31-43.Tsukahara, S., Yamakawa, H., Takai, K., Takaku, H., (1994) Nucleosides & Nucleotides, vol. 13, pp. 1617-1626.//MaeIIMethanococcus aeolicus PL-15/HHpyCH4IV,HpyF13III,HpyF35II,HpyF74II,TaiI,TscI,Tsp49I,TspIDSI,TspWAM8AI,TtmIK.O. StetterM1Schmid, K., Thomm, M., Laminet, A., Laue, F.G., Kessler, C., Stetter, K.O., Schmitt, R., (1984) Nucleic Acids Res., vol. 12, pp. 2619-2628.//NotINocardia otitidis-caviarumCciNI,CspBI,MchAI?(4)ATCC 14630ABCDEFGHJKLMNOQRSTU4Borsetti, R., Wise, D., Qiang, B.-Q., Schildkraut, I., Unpublished observations.Morgan, R.D., Unpublished observations.Morgan, R.D., Benner, J.S., Claus, T.E., US Patent Office, 1994.Qiang, B.-Q., Schildkraut, I., (1987) Methods Enzymol., vol. 155, pp. 15-21.//TaqIThermus aquaticus YTICviSIII,EsaBC3I,HpyV,Hpy26II,HpyF14III,HpyF16I,HpyF23I,HpyF24I,HpyF26III,HpyF30I,HpyF35I,HpyF40II,HpyF42IV,HpyF45I,HpyF49I,HpyF52I,HpyF59III,HpyF62II,HpyF64I,HpyF65II,HpyF66IV,HpyF71I,HpyF73II,HpyJP26II,PpaAII,Taq20I,Tbr51I,TfiA3I,TfiTok4A2I,TfiTok6A1I,TflI,Tsc4aI,Tsp32I,Tsp32II,Tsp358I,Tsp505I,Tsp510I,TspAK13D21I,TspAK16D24I,TspNI,TspVi4AI,TspVil3I,Tth24I,TthHB8I,TthRQI4(6)J.I. HarrisABCDEFGIJLMNOQRSTU5Anton, B.P., Brooks, J.E., Unpublished observations.Fomenkov, A., Xiao, J.-P., Dila, D., Raleigh, E., Xu, S.-Y., (1994) Nucleic Acids Res., vol. 22, pp. 2399-2403.McClelland, M., (1981) Nucleic Acids Res., vol. 9, pp. 6795-6804.Sato, S., Hutchison, C.A. III, Harris, J.I., (1977) Proc. Natl. Acad. Sci. U. S. A., vol. 74, pp. 542-546.Zebala, J.A., (1993) Diss. Abstr., vol. 54, pp. 1394-1398.// File: REBASE/embossre.sup# REBASE Supplier information for EMBOSS## Format:# Code of Supplier<ws>Supplier name#A Amersham Pharmacia Biotech (1/01)B Life Technologies Inc. (1/98)C Minotech, Molecular Biology Products (12/00)D HYBAID GmbH (12/00)E Stratagene (11/00)F Fermentas AB (5/01)G Q-BIOgene (1/01)H American Allied Biochemical, Inc. (10/98)I SibEnzyme Ltd. (1/01)J Nippon Gene Co., Ltd. (6/00)K Takara Shuzo Co. Ltd. (2/01)L Transgenomic Ltd. (1/01)M Roche Molecular Biochemicals (1/01)N New England BioLabs (12/00)O Toyobo Biochemicals (11/98)P Megabase Research Products (5/99)Q CHIMERx (10/97)R Promega Corporation (6/99)S Sigma Chemical Corporation (11/98)T Advanced Biotechnologies Ltd. (3/98)U Bangalore Genei (2/01)V MRC-Holland (3/01) The output files are held in the REBASE subdirectory of the EMBOSS data directory. There are three: * embossre.enz Enzyme pattern file * embossre.ref Enzyme references * embossre.sup Enzyme suppliers rebaseextract will also (by default) produce an 'embossre.equ' file in the EMBOSS data directory. This can be turned off by setting the -equivalences option to be false. This option calculates an 'embossre.equ' file using restriction enzyme prototypes in the "withrefm" file. The 'embossre.equ' file is a file of preferred isoschizomers. You may edit it to contain your available restriction enzymes.Data files The "withrefm" file of an REBASE distribution is the input file for this program.Notes The home page of REBASE is: http://rebase.neb.com/ Running this program may be the job of your system manager. The ready-made files produced by this program may already be available at the REBASE web site: http://rebase.neb.com/rebase/rebase.files.html or http://rebase.neb.com/rebase/rebase.f37.htmlReferences 1. Nucleic Acids Research 27: 312-313 (1999).Warnings The program will warn you if the input file is incorrectly formatted.Diagnostic Error MessagesExit status It exits with status 0 unless an error is reported.Known bugsSee also Program name Description aaindexextract Extract data from AAINDEX cutgextract Extract data from CUTG printsextract Extract data from PRINTS prosextract Build the PROSITE motif database for use by patmatmotifs tfextract Extract data from TRANSFACAuthor(s) Alan Bleasby (ajb
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