📄 dreg.txt
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dreg Function Regular expression search of a nucleotide sequenceDescription This searches for matches of a regular expression to a nucleic acid sequence. A regular expression is a way of specifying an ambiguous pattern to search for. Regular expressions are commonly used in some computer programming languages and may be more familiar to some users than to others. The following is a short guide to regular expressions in EMBOSS: ^ use this at the start of a pattern to insist that the pattern can only match at the start of a sequence. (eg. '^AUG' matches a start codon at the start of the sequence) $ use this at the end of a pattern to insist that the pattern can only match at the end of a sequence (eg. 'A+$' matches a poly-A sequence at the end of the sequence) () groups a pattern. This is commonly used with '|' (eg. '(AUG)|(ATG)' matches either the DNA or RNA form of the initiation codon ) | This is the OR operator to enable a match to be made to either one pattern OR another. There is no AND operator in this version of regular expressions. The following quantifier characters specify the number of time that the character before (in this case 'x') matches: x? matches 0 or 1 times (ie, '' or 'x') x* matches 0 or more times (ie, '' or 'x' or 'xx' or 'xxx', etc) x+ matches 1 or more times (ie, 'x' or 'xx' or 'xxx', etc) {min,max} Braces can enclose the specification of the minimum and maximum number of matches. A match of 'x' of between 3 and 6 times is: 'x{3,6}' Quantifiers can follow any of the following types of character specification: x any character (ie 'A') \x the character after the backslash is used instead of its normal regular expression meaning. This is commonly used to turn off the special meaning of the characters '^$()|?*+[]-.'. It may be especially useful when searching for gap characters in a sequence (eg '\.' matches only a dot character '.') [xy] match one of the characters 'x' or 'y'. You may have one or more characters in this set. [x-z] match any one of the set of characters starting with 'x' and ending in 'y' in ASCII order (eg '[A-G]' matches any one of: 'A', 'B', 'C', 'D', 'E', 'F', 'G') [^x-z] matches anything except any one of the group of characters in ASCII order (eg '[^A-G]' matches anything EXCEPT any one of: 'A', 'B', 'C', 'D', 'E', 'F', 'G') . the dot character matches any other character (eg: 'A.G' matches 'AAG', 'AaG', 'AZG', 'A-G' 'A G', etc.) Combining some of these features gives the example:'([AGC]+GGG)|(TTTGGG)' which matches one or more of any one of 'A' or 'G' or 'C' followed by three 'G's or it matches just 'TTTGGG'. Regular expressions are case-sensitive. The pattern 'AAAA' will not match the sequence 'aaaa'. For this reason, both your pattern and the input sequences are converted to upper-case.The syntax in detail EMBOSS uses the publicly available PCRE code library to do regular expressions. The full documentation of the PCRE system can be seen at http://www.pcre.org/pcre.txt A condensed description of the syntax of PCRE follows, without features that are thought not to be required for searching for patterns in sequences (e.g. matching non-printing characters, atomic grouping, back-references, assertion, conditional sub-patterns, recursive patterns, subpatterns as subroutines, callouts). If you do neot see a required function described below, please see the full description on the PCRE web site. PCRE REGULAR EXPRESSION DETAILS The syntax and semantics of the regular expressions supported by PCRE are described below. Regular expressions are also described in the Perl documentation and in a number of other books, some of which have copious examples. Jeffrey Friedl's "Mastering Regular Expressions", published by O'Reilly, covers them in great detail. The description here is intended as reference documentation. A regular expression is a pattern that is matched against a subject string from left to right. Most characters stand for themselves in a pattern, and match the corresponding characters in the subject. As a trivial example, the pattern The quick brown fox matches a portion of a subject string that is identical to itself. The power of regular expressions comes from the ability to include alternatives and repetitions in the pattern. These are encoded in the pattern by the use of meta-characters, which do not stand for themselves but instead are interpreted in some special way. There are two different sets of meta-characters: those that are recognized anywhere in the pattern except within square brackets, and those that are recognized in square brackets. Outside square brackets, the meta-characters are as follows: \ general escape character with several uses ^ assert start of string (or line, in multiline mode) $ assert end of string (or line, in multiline mode) . match any character except newline (by default) [ start character class definition | start of alternative branch ( start subpattern ) end subpattern ? extends the meaning of ( also 0 or 1 quantifier also quantifier minimizer * 0 or more quantifier + 1 or more quantifier also "possessive quantifier" { start min/max quantifier Part of a pattern that is in square brackets is called a "character class". In a character class the only meta-characters are: \ general escape character ^ negate the class, but only if the first character - indicates character range [ POSIX character class (only if followed by POSIX syntax) ] terminates the character class The following sections describe the use of each of the meta-characters. BACKSLASH The backslash character has several uses. Firstly, if it is followed by a non-alphameric character, it takes away any special meaning that character may have. This use of backslash as an escape character applies both inside and outside character classes. For example, if you want to match a * character, you write \* in the pattern. This escaping action applies whether or not the following character would otherwise be interpreted as a meta-character, so it is always safe to precede a nonalphameric with backslash to specify that it stands for itself. In particular, if you want to match a backslash, you write \\. The third use of backslash is for specifying generic character types: \d any decimal digit \D any character that is not a decimal digit \s any whitespace character \S any character that is not a whitespace character \w any "word" character W any "non-word" character Each pair of escape sequences partitions the complete set of characters into two disjoint sets. Any given character matches one, and only one, of each pair. A "word" character is any letter or digit or the underscore character, that is, any character which can be part of a Perl "word". The definition of letters and digits is controlled by PCRE's character tables, and may vary if locale- specific matching is taking place (see "Locale support" in the pcreapi page). For example, in the "fr" (French) locale, some character codes greater than 128 are used for accented letters, and these are matched by \w. These character type sequences can appear both inside and outside character classes. They each match one character of the appropriate type. If the current matching point is at the end of the subject string, all of them fail, since there is no character to match. The fourth use of backslash is for certain simple assertions. An assertion specifies a condition that has to be met at a particular point in a match, without consuming any characters from the subject string. The use of subpatterns for more complicated assertions is described below. The backslashed assertions are \b matches at a word boundary \B matches when not at a word boundary \A matches at start of subject \Z matches at end of subject or before newline at end \z matches at end of subject \G matches at first matching position in subject These assertions may not appear in character classes (but note that \b has a different meaning, namely the backspace character, inside a character class). A word boundary is a position in the subject string where the current character and the previous character do not both match \w or \W (i.e. one matches \w and the other matches \W), or the start or end of the string if the first or last character matches \w, respectively. The \A, \Z, and \z assertions differ from the traditional circumflex and dollar (described below) in that they only ever match at the very start and end of the subject string, whatever options are set. Thus, they are independent of multiline mode. CIRCUMFLEX AND DOLLAR Outside a character class, in the default matching mode, the circumflex character is an assertion which is true only if the current matching point is at the start of the subject string. Inside a character class, circumflex has an entirely different meaning (see below). Circumflex need not be the first character of the pattern if a number of alternatives are involved, but it should be the first thing in each alternative in which it appears if the pattern is ever to match that branch. If all possible alternatives start with a circumflex, that is, if the pattern is constrained to match only at the start of the subject, it is said to be an "anchored" pattern. (There are also other constructs that can cause a pattern to be anchored.) A dollar character is an assertion which is true only if the current matching point is at the end of the subject string, or immediately before a newline character that is the last character in the string (by default). Dollar need not be the last character of the pattern if a number of alternatives are involved, but it should be the last item in any branch in which it appears. Dollar has no special meaning in a character class. FULL STOP (PERIOD, DOT) Outside a character class, a dot in the pattern matches any one character in the subject, including a non-printing character, but not (by default) newline. The handling of dot is entirely independent of the handling of circumflex and dollar, the only relationship being that they both involve newline characters. Dot has no special meaning in a character class. SQUARE BRACKETS An opening square bracket introduces a character class, terminated by a closing square bracket. A closing square bracket on its own is not special. If a closing square bracket is required as a member of the class, it should be the first data character in the class (after an initial circumflex, if present) or escaped with a backslash. A character class matches a single character in the subject. A matched character must be in the set of characters defined by the class, unless the first character in the class definition is a circumflex, in which case the subject character must not be in the set defined by the class. If a circumflex is actually required as a member of the class, ensure it is not the first character, or escape it with a backslash. For example, the character class [aeiou] matches any lower case vowel, while [^aeiou] matches any character that is not a lower case vowel. Note that a circumflex is just a convenient notation for specifying the characters which are in the class by enumerating those that are not. It is not an assertion: it still consumes a character from the subject string, and fails if the current pointer is at the end of the string. When caseless matching is set, any letters in a class represent both their upper case and lower case versions, so for example, a caseless [aeiou] matches "A" as well as "a", and a caseless [^aeiou] does not match "A", whereas a caseful version would. PCRE does not support the concept of case for characters with values greater than 255. A class such as [^a] will always match a newline. The minus (hyphen) character can be used to specify a range of characters in a character class. For example, [d-m] matches any letter between d and m, inclusive. If a minus character is required in a class, it must be escaped with a backslash or appear in a position where it cannot be interpreted as indicating a range, typically as the first or last character in the class. It is not possible to have the literal character "]" as the end character of a range. A pattern such as [W-]46] is interpreted as a class of two characters ("W" and "-") followed by a literal string "46]", so it would match "W46]" or "-46]". However, if the "]" is escaped with a backslash it is interpreted as the end of range, so [W-\]46] is interpreted as a single class containing a range followed by two separate characters. The octal or hexadecimal representation of "]" can also be used to end a range. The character types \d, \D, \s, \S, \w, and \W may also appear in a character class, and add the characters that they match to the class. For example, [\dABCDEF] matches any hexadecimal digit. A circumflex can conveniently be used with the upper case character types to specify a more restricted set of characters than the matching lower case type. For example, the class [^\W_] matches any letter or digit,
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