📄 newseq.txt
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newseq Function Type in a short new sequenceDescription This allows you to type a sequence into a file in a quick and easy manner. The length of the sequence you can type in is restricted to a fairly short length (typically less than 255 characters). This length restriction is not a property of the EMBOSS package, but of the computer system you are using. This is because, as you type in response to a prompt from this program, what you type is stored in the computer operating system before being handed over to the program. There is often a limit of less than 255 characters on the length of a response that a computer system will allow you to give. Despite this restriction, it is expected that this program will be a useful and easy way of constructing new sequence files. (You wouldn't want to type a long sequence in by hand, anyway, would you?)Usage Here is a sample session with newseq Type in a short sequence to the file 'mycc.pep' in SWISSPROT format:% newseq Type in a short new sequence.Name of the sequence: cytocDescription of the sequence: fragment of cytochrome cType of sequence N : Nucleic P : ProteinType of sequence [N]: pEnter the sequence: KKKEERADLIAYoutput sequence [outfile.fasta]: swiss::mycc.pep Go to the output files for this exampleCommand line arguments Standard (Mandatory) qualifiers: [-name] string The name of of the sequence should be a single word that you will use to identify the sequence. It should have no (or few) punctuation characters in it. (Any string is accepted) [-description] string Enter any description of the sequence that you require. (Any string is accepted) [-type] menu [N] Type of sequence (Values: N (Nucleic); P (Protein)) [-sequence] string The sequence itself. Because of the limitation of the operating system, you will only be able to type in a short sequence of (typically) 250 characters, or so. The keyboard will beep at you when you have reached this limit and you will not be able to press the RETURN/ENTER key until you have deleted a few characters. (Any string is accepted) [-outseq] seqout [.] Sequence filename and optional format (output USA) Additional (Optional) qualifiers: (none) Advanced (Unprompted) qualifiers: (none) Associated qualifiers: "-outseq" associated qualifiers -osformat5 string Output seq format -osextension5 string File name extension -osname5 string Base file name -osdirectory5 string Output directory -osdbname5 string Database name to add -ossingle5 boolean Separate file for each entry -oufo5 string UFO features -offormat5 string Features format -ofname5 string Features file name -ofdirectory5 string Output directory General qualifiers: -auto boolean Turn off prompts -stdout boolean Write standard output -filter boolean Read standard input, write standard output -options boolean Prompt for standard and additional values -debug boolean Write debug output to program.dbg -verbose boolean Report some/full command line options -help boolean Report command line options. More information on associated and general qualifiers can be found with -help -verbose -warning boolean Report warnings -error boolean Report errors -fatal boolean Report fatal errors -die boolean Report dying program messagesInput file formatOutput file format newseq writes a normal sequence file. Output files for usage example File: mycc.pepID cytoc STANDARD; PRT; 12 AA.DE fragment of cytochrome cSQ SEQUENCE 12 AA; 1464 MW; 6F142FA88DADC40B CRC64; KKKEERADLI AY//Data files None.Notes The length of the sequence you can type in is restricted to a fairly short length (typically less than 255 characters). If your computer beeps at you, you will typically have to delete the last character you typed and press the RETURN key.References None.Warnings None.Diagnostic Error Messages None.Exit status It always exits with status 0.Known bugs None.See also Program name Description biosed Replace or delete sequence sections codcopy Reads and writes a codon usage table cutseq Removes a specified section from a sequence degapseq Removes gap characters from sequences descseq Alter the name or description of a sequence entret Reads and writes (returns) flatfile entries extractalign Extract regions from a sequence alignment extractfeat Extract features from a sequence extractseq Extract regions from a sequence listor Write a list file of the logical OR of two sets of sequences makenucseq Creates random nucleotide sequences makeprotseq Creates random protein sequences maskfeat Mask off features of a sequence maskseq Mask off regions of a sequence noreturn Removes carriage return from ASCII files notseq Exclude a set of sequences and write out the remaining ones nthseq Writes one sequence from a multiple set of sequences pasteseq Insert one sequence into another revseq Reverse and complement a sequence seqret Reads and writes (returns) sequences seqretsplit Reads and writes (returns) sequences in individual files skipseq Reads and writes (returns) sequences, skipping first few splitter Split a sequence into (overlapping) smaller sequences trimest Trim poly-A tails off EST sequences trimseq Trim ambiguous bits off the ends of sequences union Reads sequence fragments and builds one sequence vectorstrip Strips out DNA between a pair of vector sequences yank Reads a sequence range, appends the full USA to a list fileAuthor(s) Gary Williams (gwilliam
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