📄 equicktandem.txt
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equicktandem Function Finds tandem repeatsDescription equicktandem scans a sequence for potential tandem repeats up to a specified size. The results can be used to run etandem on the candidate repeat lengths to identify genuine tandem repeats. equicktandem is a simple program that looks for segments in which each base tends to match the base 'repeatsize' back, i.e. with an autocorrelation peak at 'repeatsize'. This can allow drift in the repeating sequence, i.e. it does not look for a consensus sequence for the whole repeat block (that is what etandem does). But it is much quicker than etandem. It does not account for gaps. The score is +1 for a match to the corresponding base 'repeatsize' back and -1 for a mismatch.Usage Here is a sample session with equicktandem The input sequence is the human herpesvirus tandem repeat.% equicktandem -noorigfile Finds tandem repeatsInput nucleotide sequence: tembl:hhtetraMaximum repeat size [600]: Threshold score [20]: Output report [hhtetra.qtan]: Go to the input files for this example Go to the output files for this exampleCommand line arguments Standard (Mandatory) qualifiers: [-sequence] sequence Nucleotide sequence filename and optional format, or reference (input USA) -maxrepeat integer [600] Maximum repeat size (Any integer value) -threshold integer [20] Threshold score (Any integer value) [-outfile] report [*.equicktandem] Output report file name Additional (Optional) qualifiers: (none) Advanced (Unprompted) qualifiers: -origfile outfile [*.equicktandem] Sanger Centre program quicktandem output file (optional) Associated qualifiers: "-sequence" associated qualifiers -sbegin1 integer Start of the sequence to be used -send1 integer End of the sequence to be used -sreverse1 boolean Reverse (if DNA) -sask1 boolean Ask for begin/end/reverse -snucleotide1 boolean Sequence is nucleotide -sprotein1 boolean Sequence is protein -slower1 boolean Make lower case -supper1 boolean Make upper case -sformat1 string Input sequence format -sdbname1 string Database name -sid1 string Entryname -ufo1 string UFO features -fformat1 string Features format -fopenfile1 string Features file name "-outfile" associated qualifiers -rformat2 string Report format -rname2 string Base file name -rextension2 string File name extension -rdirectory2 string Output directory -raccshow2 boolean Show accession number in the report -rdesshow2 boolean Show description in the report -rscoreshow2 boolean Show the score in the report -rusashow2 boolean Show the full USA in the report -rmaxall2 integer Maximum total hits to report -rmaxseq2 integer Maximum hits to report for one sequence "-origfile" associated qualifiers -odirectory string Output directory General qualifiers: -auto boolean Turn off prompts -stdout boolean Write standard output -filter boolean Read standard input, write standard output -options boolean Prompt for standard and additional values -debug boolean Write debug output to program.dbg -verbose boolean Report some/full command line options -help boolean Report command line options. More information on associated and general qualifiers can be found with -help -verbose -warning boolean Report warnings -error boolean Report errors -fatal boolean Report fatal errors -die boolean Report dying program messagesInput file format The input for equicktandem is a nucleotide sequence USA. Input files for usage example 'tembl:hhtetra' is a sequence entry in the example nucleic acid database 'tembl' Database entry: tembl:hhtetraID HHTETRA standard; DNA; VRL; 1272 BP.XXAC L46634; L46689;XXSV L46634.1XXDT 06-NOV-1995 (Rel. 45, Created)DT 04-MAR-2000 (Rel. 63, Last updated, Version 3)XXDE Human herpesvirus 7 (clone ED132'1.2) telomeric repeat region.XXKW telomeric repeat.XXOS Human herpesvirus 7OC Viruses; dsDNA viruses, no RNA stage; Herpesviridae; Betaherpesvirinae.XXRN [1]RP 1-1272RX MEDLINE; 96079055.RA Secchiero P., Nicholas J., Deng H., Xiaopeng T., van Loon N., Ruvolo V.R.,RA Berneman Z.N., Reitz M.S. Jr., Dewhurst S.;RT "Identification of human telomeric repeat motifs at the genome termini ofRT human herpesvirus 7: structural analysis and heterogeneity";RL J. Virol. 69(12):8041-8045(1995).XXFH Key Location/QualifiersFHFT source 1..1272FT /db_xref="taxon:10372"FT /organism="Human herpesvirus 7"FT /strain="JI"FT /clone="ED132'1.2"FT repeat_region 207..928FT /note="long and complex repeat region composed of variousFT direct repeats, including TAACCC (TRS), degenerate copiesFT of TRS motifs and a 14-bp repeat, TAGGGCTGCGGCCC"FT misc_signal 938..998FT /note="pac2 motif"FT misc_feature 1009FT /note="right genome terminus (...ACA)"XXSQ Sequence 1272 BP; 346 A; 455 C; 222 G; 249 T; 0 other; aagcttaaac tgaggtcaca cacgacttta attacggcaa cgcaacagct gtaagctgca 60 ggaaagatac gatcgtaagc aaatgtagtc ctacaatcaa gcgaggttgt agacgttacc 120 tacaatgaac tacacctcta agcataacct gtcgggcaca gtgagacacg cagccgtaaa 180 ttcaaaactc aacccaaacc gaagtctaag tctcacccta atcgtaacag taaccctaca 240 actctaatcc tagtccgtaa ccgtaacccc aatcctagcc cttagcccta accctagccc 300 taaccctagc tctaacctta gctctaactc tgaccctagg cctaacccta agcctaaccc 360 taaccgtagc tctaagttta accctaaccc taaccctaac catgaccctg accctaaccc 420 tagggctgcg gccctaaccc tagccctaac cctaacccta atcctaatcc tagccctaac 480 cctagggctg cggccctaac cctagcccta accctaaccc taaccctagg gctgcggccc 540 taaccctaac cctagggctg cggcccgaac cctaacccta accctaaccc taaccctagg 600 gctgcggccc taaccctaac cctagggctg cggccctaac cctaacccta gggctgcggc 660 ccgaacccta accctaaccc taaccctagg gctgcggccc taaccctaac cctagggctg 720 cggccctaac cctaacccta actctagggc tgcggcccta accctaaccc taaccctaac 780 cctagggctg cggcccgaac cctagcccta accctaaccc tgaccctgac cctaacccta 840 accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta 900 accctaaccc taaccctaac cctaaccccg cccccactgg cagccaatgt cttgtaatgc 960 cttcaaggca ctttttctgc gagccgcgcg cagcactcag tgaaaaacaa gtttgtgcac 1020 gagaaagacg ctgccaaacc gcagctgcag catgaaggct gagtgcacaa ttttggcttt 1080 agtcccataa aggcgcggct tcccgtagag tagaaaaccg cagcgcggcg cacagagcga 1140 aggcagcggc tttcagactg tttgccaagc gcagtctgca tcttaccaat gatgatcgca 1200 agcaagaaaa atgttctttc ttagcatatg cgtggttaat cctgttgtgg tcatcactaa 1260 gttttcaagc tt 1272//Output file format The output is a standard EMBOSS report file. The results can be output in one of several styles by using the command-line qualifier -rformat xxx, where 'xxx' is replaced by the name of the required format. The available format names are: embl, genbank, gff, pir, swiss, trace, listfile, dbmotif, diffseq, excel, feattable, motif, regions, seqtable, simple, srs, table, tagseq See: http://emboss.sf.net/docs/themes/ReportFormats.html for further information on report formats. By default equicktandem writes a 'table' report file. Output files for usage example File: hhtetra.qtan######################################### Program: equicktandem# Rundate: Sat 15 Jul 2006 12:00:00# Commandline: equicktandem# -noorigfile# -sequence tembl:hhtetra# Report_format: table# Report_file: hhtetra.qtan#########################################=======================================## Sequence: HHTETRA from: 1 to: 1272# HitCount: 1## Threshold: 20# Maxrepeat: 600##======================================= Start End Score Size Count 191 935 339 6 124#---------------------------------------#---------------------------------------Data files None.Notes None.References None.Warnings None.Diagnostic Error Messages None.Exit status It always exits with a status of 0.Known bugs None.See also Program name Description einverted Finds DNA inverted repeats etandem Looks for tandem repeats in a nucleotide sequence palindrome Looks for inverted repeats in a nucleotide sequence equicktandem identifies regions wich are likely to contain tanden repeats. tandem should then be run on those regions to confirm them and to get an accurate specification of the repeats. tandem runs slowly.Author(s) This program was originally written by Richard Durbin (rd
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