38.txt

来自「the test core for find word in xxx.txt」· 文本 代码 · 共 57 行

1: Mol Reprod Dev. 2006 May;73(5):540-50.

FGF ligand family mRNA expression profile for mouse preimplantation embryos,
early gestation human placenta, and mouse trophoblast stem cells.

Zhong W, Wang QT, Sun T, Wang F, Liu J, Leach R, Johnson A, Puscheck EE, Rappolee
DA.

Department of Anatomy and Cell Biology, Wayne State University School of
Medicine, Detroit, Michigan 48201, USA.

Signaling by fibroblast growth factor (FGF) is essential is for trophoblast stem 
(TS) cells and preimplantation embryos. FGF4 provides essential signaling, but
the expression of the complete set of 23 FGF family members has not been
analyzed. Here, semi-quantitative RT-PCR and microarray analyses were used to
define expression of all FGF ligand mRNA. RT-PCR was done for developmentally
important FGF subfamilies, FGF10/FGF22 and FGF8/FGF17/FGF18 as well as FGF11.
FGF4 and FGF18 are detected at highest levels by RT-PCR and microarrays. FGF10
was detected at low levels in both assays. FGF11 was detected at moderate levels 
by microarray, but not by RT-PCR. FGF17 was detected at low levels by array and
moderate levels by RT-PCR. FGF8 and FGF22 were detected by RT-PCR, but not by
microarrays during late cleavage divisions. FGF8, FGF5, and FGF9 were detected in
the oocyte by microarray. FGF2, FGF3, and FGF7 were not detected by RT-PCR or
microarrays and FGF13, FGF14, and FGF23 were not detected by microarray. Since a 
major role of FGF is to maintain TS cells, we tested human and mouse placental
cell lines and early gestation human placenta for expression of FGF ligands.
Expression in mouse TS cells was compared with preimplantation embryos, and human
placental cell line expression was compared with human placenta, to infer which
ligands are expressed in placental lineage vs. other cell lineages. The data
suggest that human and mouse placenta share FGF18 and its high expression
suggests preimplantation and early placental function. Mol. Reprod. Dev. (c) 2006
Wiley-Liss, Inc.

PMID: 16470835 [PubMed - indexed for MEDLINE]

Related Links

    SAPKgamma/JNK1 and SAPKalpha/JNK2 mRNA transcripts are expressed in early
gestation human placenta and mouse eggs, preimplantation embryos, and trophoblast
stem cells. [Fertil Steril. 2004] PMID:15474087

    Fibroblast growth factor (FGF)-1, FGF2, FGF7 and FGF receptors are uniformly
expressed in trophoblast giant cells during restricted trophoblast invasion in
cows. [Placenta. 2006] PMID:16129484

    Six post-implantation lethal knockouts of genes for lipophilic MAPK pathway
proteins are expressed in preimplantation mouse embryos and trophoblast stem
cells. [Mol Reprod Dev. 2005] PMID:15736129

    Expression pattern of fibroblast growth factors (FGFs), their receptors and
antagonists in primary endothelial cells and vascular smooth muscle cells.
[Growth Factors. 2005] PMID:16019430

    PLAC1, a trophoblast-specific gene, is expressed throughout pregnancy in the
human placenta and modulated by keratinocyte growth factor. [Mol Reprod Dev.
2002] PMID:12412044