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1: Genesis. 2006 Mar;44(3):150-4.

Generation of an Fgf9 conditional null allele.

Lin Y, Liu G, Wang F.

Center for Cancer Biology and Nutrition, Institute of Biosciences and Technology,
Texas A&M University System Health Science Center, Houston, Texas 77030-3303,
USA.

The fibroblast growth factor (FGF) family consists of 22 widely expressed
regulatory polypeptides and controls a broad spectrum of cellular processes.
Accumulating data show that FGF9 plays important roles both in embryogenesis and 
in adult tissue homeostasis. Ablation of Fgf9 alleles leads to lethality at the
neonatal stage mainly due to malformations of the lung, as well as causing
male-to-female sex reversal. To circumvent the neonatal lethality resulting from 
disruption of the Fgf9 gene, which hinders further characterization of the role
of FGF9 in adult tissue function and homeostasis, we generated an Fgf9
conditional null allele for spatiotemporal- and tissue-specific disruption of
Fgf9. Using gene targeting in mouse embryonic stem (ES) cells, we introduced two 
loxP sites flanking exon 1 in the Fgf9 allele, which encodes 93 amino acid
residues at the N-terminal of FGF9. Our results indicate that the Fgf9
conditional null allele is a true conditional null that encodes wildtype activity
and reverts to a null allele after recombination mediated by the Cre recombinase.
(c) 2006 Wiley-Liss, Inc.

PMID: 16496342 [PubMed - indexed for MEDLINE]

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