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1: J Biol Chem. 2006 Dec 1;281(48):36905-14. Epub 2006 Sep 19.

The structure, location, and function of perlecan, a prominent pericellular
proteoglycan of fetal, postnatal, and mature hyaline cartilages.

Melrose J, Roughley P, Knox S, Smith S, Lord M, Whitelock J.

The Raymond Purves Research Laboratories, Institute of Bone and Joint ,
University of Sydney at the Royal North Shore Hospital of Sydney, St. Leonards,
New South Wales 2065, Australia. [e-mail]

The aim of this study was to immunolocalize perlecan in human fetal, postnatal,
and mature hyaline cartilages and to determine information on the structure and
function of chondrocyte perlecan. Perlecan is a prominent component of human
fetal (12-14 week) finger, toe, knee, and elbow cartilages; it was localized
diffusely in the interterritorial extracellular matrix, densely in the
pericellular matrix around chondrocytes, and to small blood vessels in the joint 
capsules and perichondrium. Aggrecan had a more intense distribution in the
marginal regions of the joint rudiments and in para-articular structures.
Perlecan also had a strong pericellular localization pattern in postnatal (2-7
month) and mature (55-64 year) femoral cartilages, whereas aggrecan had a
prominent extracellular matrix distribution in these tissues. Western blotting
identified multiple perlecan core protein species in extracts of the postnatal
and mature cartilages, some of which were substituted with heparan sulfate and/or
chondroitin sulfate and some were devoid of glycosaminoglycan substitution. Some 
perlecan core proteins were smaller than intact perlecan, suggesting that
proteolytic processing or alternative splicing had occurred. Surface plasmon
resonance and quartz crystal microbalance with dissipation experiments
demonstrated that chondrocyte perlecan bound fibroblast growth factor (FGF)-1 and
-9 less efficiently than endothelial cell perlecan. The latter perlecan supported
the proliferation of Baf-32 cells transfected with FGFR3c equally well with FGF-1
and -9, whereas chondrocyte perlecan only supported Baf-32 cell proliferation
with FGF-9. The function of perlecan therefore may not be universal but may vary 
with its cellular origin and presumably its structure.

PMID: 16984910 [PubMed - indexed for MEDLINE]

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