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1: Mol Reprod Dev. 2007 Dec;74(12):1491-504.

Presumptive pre-sertoli cells express genes involved in cell proliferation and
cell signalling during a critical window in early testis differentiation.

Cory AT, Boyer A, Pilon N, Lussier JG, Silversides DW.

Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Quebec, 
Canada.

In mammals, the pre-Sertoli cell of the male genital ridge is the first cell type
to display sex specific differentiation and differential gene expression. The
genetic cascade driving the differentiationof pre-Sertoli cells and ultimately
testis formationis beginning to be unravelled, but many questions remain. A
better understanding of the transcriptome of pre-Sertoli cells immediately after 
sex determination is essential in order to further understand this
differentiationprocess. A mouse model expressing Red Fluorescent Protein (RFP)
under the control of a hybrid mouse/pig SRY promoter (HybSRYp-RFP) was used to
purify cells from embryonic day 12.0 (e12.0) male genital ridges. To compare the 
transcriptomes of HybSRYp-RFP cell populations versus age matched whole female
genital ridges, RNA was extracted and used to generate molecular probes that were
hybridized onto Affymetrix Mouse Genome 430 2.0 micro-arrays. The expression of
genes considered markers for pre-Sertoli cells, including Sox9, Mis, Dhh and Fgf9
were identified within the HybSRYp-RFP expressing cell population, while markers 
for germ cells (Oct4, SSEA-1) and endothelial cells (Ntrk3) were not identified. 
In contrast, markers for ovarian somatic cell expression, including Fst and Bmp2,
were identified as overexpressed within the ovarian cell population. In a general
fashion, genes identified as 2.5-fold over expressed in HybSRYp-RFP expressing
cells coded notably for cell signalling and extra cellular proteins. The
expression of Sox10, Stc2, Fgf18, Fgf13 and Wnt6 were further characterized via
whole mount in situ hybridization (WISH) on male and female genital ridges
between e11.5 and e14.5. Sox10, Fgf18, Fgf13 and Stc2 gene expression was
detected within the male genital ridges while Wnt6 was found diffusely within
both the male and female genital ridges. These data represent the earliest
comprehensive microarray expression analysis of purified presumptive pre-Sertoli 
cells available to date. Mol. Reprod. Dev. 74: 1491-1504, 2007. (c) 2007
Wiley-Liss, Inc.

PMID: 17410545 [PubMed - in process]

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