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1: Hum Reprod. 2006 Jul;21(7):1705-19. Epub 2006 Mar 29.

The human cumulus--oocyte complex gene-expression profile.

Assou S, Anahory T, Pantesco V, Le Carrour T, Pellestor F, Klein B, Reyftmann L, 
Dechaud H, De Vos J, Hamamah S.

CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, and
INSERM U 475, Université Montpellier, UFR de Médecine, France.

BACKGROUND: The understanding of the mechanisms regulating human oocyte
maturation is still rudimentary. We have identified transcripts differentially
expressed between immature and mature oocytes and cumulus cells. METHODS: Using
oligonucleotide microarrays, genome-wide gene expression was studied in pooled
immature and mature oocytes or cumulus cells from patients who underwent IVF.
RESULTS: In addition to known genes, such as DAZL, BMP15 or GDF9, oocytes
up-regulated 1514 genes. We show that PTTG3 and AURKC are respectively the
securin and the Aurora kinase preferentially expressed during oocyte meiosis.
Strikingly, oocytes overexpressed previously unreported growth factors such as
TNFSF13/APRIL, FGF9, FGF14 and IL4 and transcription factors including OTX2,
SOX15 and SOX30. Conversely, cumulus cells, in addition to known genes such as
LHCGR or BMPR2, overexpressed cell-to-cell signalling genes including
TNFSF11/RANKL, numerous complement components, semaphorins (SEMA3A, SEMA6A and
SEMA6D) and CD genes such as CD200. We also identified 52 genes progressively
increasing during oocyte maturation, including CDC25A and SOCS7. CONCLUSION: The 
identification of genes that were up- and down-regulated during oocyte maturation
greatly improves our understanding of oocyte biology and will provide new markers
that signal viable and competent oocytes. Furthermore, genes found expressed in
cumulus cells are potential markers of granulosa cell tumours.

PMID: 16571642 [PubMed - indexed for MEDLINE]

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