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1: Chin Med J (Engl). 2007 Mar 20;120(6):491-5.

Effect of fibroblast growth factor 9 on Runx2 gene promoter activity in MC3T3-E1 
and C2C12 cells.

Yu LY, Pei Y, Xia WB, Xing XP, Meng XW, Zhou XY.

Department of Anesthesiology, Beijing Electric Power Hospital, Beijing 100073,
China.

BACKGROUND: Fibroblast growth factor 9 (FGF9), expressed in brain, kidney and
developing skeletal tissues, can physiologically inhibit endochondral
ossification; but little is known about how FGF9 affects osteoblasts and its
detailed regulatory mechanism. Here we examined the effect of FGF9 on the
activity of the murine Runt-related transcription factor 2 (Runx2) gene promoter 
in preosteoblast MC3T3-E1 and premyoblast C2C12 cells. METHODS: Plasmids
containing the Runx2 promoter region were transfected into MC3T3-E1 and C2C12
cells and stably transfected cell lines were established. The method of
luciferase reporter gene activation was used to examine the effects of FGF9 on
the promoter activity. RESULTS: FGF9 (10 ng/ml) increased Runx2 promoter activity
in MC3T3-E1 cells. When MC3T3-E1 cells were treated with FGF9 plus the various
inhibitors or activator of the intracellular signaling transducation pathways,
including 10 micromol/L U0126 (the inhibitor of mitogen-activated protein kinase 
kinase), 10 micromol/L SB203580 (the inhibitor of p38/mitogen activated protein
kinase), or 1 micromol/L C6 ceramide (an activator of mitogen activated protein
kinase), the luciferase expression did not change significantly compared with
that of the cells treated with FGF9 only. However, when C2C12 cells were treated 
with 10 ng/ml FGF9, Runx2 gene promoter activity first decreased and then
increased over a period of 1 to 5 days. Among the above inhibitors, only U0126
(10 micromol/L) completely blocked the effects of FGF9 on Runx2 gene promoter
activity. CONCLUSIONS: Our data showed that FGF9 can affect Runx2 gene promoter
activity in MC3T3-E1 and C2C12 cells. The action of FGF9 appears to depend partly
on the mitogen-activated protein kinase kinase/mitogen-activated protein kinase
pathways in C2C12 cells.

PMID: 17439743 [PubMed - indexed for MEDLINE]

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