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Data Arangement: Y ------------------ (X) A C G U ------------------ 5' ==> 3' AX AY 3' <== 5' (A) . . . . (C) . . . . (G) . . . . (U) -0.70 -0.10 -0.70 -0.10 -------------------------------- STACKING ENTHALPIES : TERMINAL MISMATCHES Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' AX AX AX AX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . -4.00 . . . -4.30 . . . -3.80 -4.30 -6.00 -6.00 -6.00 . . -5.20 . . . -7.20 . . . -7.10 . -2.60 -2.40 -2.40 -2.40 . -10.30 . -7.20 . -5.20 . -4.80 . -9.40 . -6.60 -3.40 -6.90 -6.90 -6.90 -4.30 . -4.30 . -2.60 . -2.60 . -3.40 . -3.40 . -3.30 -3.30 -3.30 -3.30 Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' CX CX CX CX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . -6.30 . . . -5.10 -10.30-9.50 -10.30-10.30 . . . -1.40 . . -8.80 . . . -3.10 . -5.20 -4.50 -5.20 -6.70 . . -5.00 . . -9.50 . -7.90 . -4.50 . -4.80 -9.40 -9.40 -9.40 -9.40 . -7.40 . -4.40 -6.00 . -6.00 . -2.40 . -2.40 . -8.10 -7.40 -8.10 -8.60 -3.30 . -3.30 . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' GX GX GX GX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . -8.90 -5.20 -8.80 -5.60 -8.80 . . . -8.90 -4.30 -6.00 -6.00 -6.00 . . -5.60 . -7.20 -3.10 -3.10 -3.90 . . -6.20 . -2.60 -2.40 -2.40 -2.40 . -10.30 . -9.60 -7.10 -7.40 -6.20 -7.40 . -9.40 . -9.20 -3.40 -6.90 -6.90 -6.90 -6.00 . -6.00 . -5.00 -5.00 -5.00 -5.70 -6.90 . -6.90 . -3.30 -3.30 -3.30 -3.30 Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' UX UX UX UX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -4.00 -6.30 -8.90 -5.90 . . . -1.80 -7.20 -7.90 -9.60 -8.10 . . . 1.40 -4.30 -5.10 -2.00 -1.80 . . -3.90 . -4.80 -4.80 -3.60 -4.80 . . -5.70 . -3.80 -6.80 -8.90 -6.80 . -6.70 . -4.80 -6.60 -8.10 -9.20 -8.10 . -8.60 . -3.60 -2.80 -1.40 -2.80 -1.40 -2.40 . -2.40 . -5.50 -4.40 -5.50 -3.60 -3.30 . -3.30 .
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