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Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G T A C G T A C G T A C G T A C G T A C G T ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C T C G C T G T G G G C G A G T G G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -2.45 -2.55 -2.48 -4.09 -3.00 -3.10 -3.03 -4.64 -3.03 -3.13 -3.06 -4.68 -2.45 -2.55 -2.48 -4.09 -1.90 -2.00 -1.93 -3.55 -1.90 -2.00 -1.93 -3.55 -2.61 -2.68 -3.87 -2.61 -3.16 -3.22 -4.42 -3.16 -3.19 -3.26 -4.45 -3.19 -2.61 -2.68 -3.87 -2.61 -2.00 -2.06 -3.26 -2.00 -2.00 -2.06 -3.26 -2.00 -4.48 -6.55 -4.48 -4.64 -4.74 -6.80 -4.74 -4.90 -4.32 -6.38 -4.32 -4.48 -4.58 -6.64 -4.58 -4.74 -0.84 -2.90 -0.84 -1.00 -0.45 -2.51 -0.45 -0.61 -3.97 -2.58 -2.29 -2.51 -4.51 -3.13 -2.84 -3.06 -4.51 -3.13 -2.84 -3.06 -3.97 -2.58 -2.29 -2.51 -3.39 -2.00 -1.71 -1.93 -3.39 -2.00 -1.71 -1.93 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G T A C G T A C G T A C G T A C G T A C G T ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C T C G C T G T G G G C G A G T G G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -2.42 -2.51 -2.45 -4.06 -2.93 -3.03 -2.97 -4.58 -2.97 -3.06 -3.00 -4.61 -2.42 -2.51 -2.45 -4.06 -1.81 -1.90 -1.84 -3.45 -1.81 -1.90 -1.84 -3.45 -4.80 -4.87 -6.06 -4.80 -4.38 -4.45 -5.64 -4.38 -5.13 -5.19 -6.38 -5.13 -4.51 -4.58 -5.77 -4.51 -3.22 -3.29 -4.48 -3.22 -2.84 -2.90 -4.09 -2.84 -2.45 -4.51 -2.45 -2.61 -2.97 -5.03 -2.97 -3.13 -3.00 -5.06 -3.00 -3.16 -2.45 -4.51 -2.45 -2.61 -1.84 -3.90 -1.84 -2.00 -1.84 -3.90 -1.84 -2.00 -3.48 -2.10 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-0.13 -2.19 -0.13 -0.29 -0.68 -2.74 -0.68 -0.84 -3.90 -2.51 -2.22 -2.45 -4.45 -3.06 -2.77 -3.00 -4.45 -3.06 -2.77 -3.00 -3.90 -2.51 -2.22 -2.45 -3.39 -2.00 -1.71 -1.93 -3.39 -2.00 -1.71 -1.93 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G T A C G T A C G T A C G T A C G T A C G T ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G T G G G T C T C G C C C A C T C G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -2.39 -2.48 -2.42 -3.93 -2.87 -2.97 -2.90 -4.42 -2.90 -3.00 -2.93 -4.45 -2.35 -2.45 -2.39 -3.90 -1.77 -1.87 -1.81 -3.32 -1.77 -1.87 -1.81 -3.32 -2.51 -2.58 -4.22 -2.55 -3.06 -3.13 -4.77 -3.10 -3.10 -3.16 -4.80 -3.13 -2.51 -2.58 -4.22 -2.55 -1.97 -2.03 -3.68 -2.00 -1.97 -2.03 -3.68 -2.00 -2.42 -3.77 -2.42 -0.29 -2.93 -4.29 -2.93 -0.81 -2.97 -4.32 -2.97 -0.84 -2.42 -3.77 -2.42 -0.29 -1.81 -3.16 -1.81 0.32 -1.81 -3.16 -1.81 0.32 -5.90 -4.38 -4.61 -4.32 -6.06 -4.55 -4.77 -4.48 -6.19 -4.68 -4.90 -4.61 -6.13 -4.61 -4.84 -4.55 -3.61 -2.10 -2.32 -2.03 -3.93 -2.42 -2.64 -2.35 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G T A C G T A C G T A C G T A C G T A C G T ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G T G G G T C T C G C C C A C T C G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -2.42 -2.51 -2.45 -3.97 -2.97 -3.06 -3.00 -4.51 -3.00 -3.10 -3.03 -4.55 -2.42 -2.51 -2.45 -3.97 -1.87 -1.97 -1.90 -3.42 -1.87 -1.97 -1.90 -3.42 -2.58 -2.64 -4.29 -2.61 -3.13 -3.19 -4.84 -3.16 -3.16 -3.22 -4.87 -3.19 -2.58 -2.64 -4.29 -2.61 -1.97 -2.03 -3.68 -2.00 -1.97 -2.03 -3.68 -2.00 -4.45 -5.80 -4.45 -2.32 -4.71 -6.06 -4.71 -2.58 -4.29 -5.64 -4.29 -2.16 -4.55 -5.90 -4.55 -2.42 -0.81 -2.16 -0.81 1.32 -0.42 -1.77 -0.42 1.71 -4.09 -2.58 -2.81 -2.51 -4.64 -3.13 -3.35 -3.06 -4.64 -3.13 -3.35 -3.06 -4.09 -2.58 -2.81 -2.51 -3.51 -2.00 -2.22 -1.93 -3.51 -2.00 -2.22 -1.93 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G T A C G T A C G T A C G T A C G T A C G T ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G T G G G T C T C G C C C A C T C G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -2.39 -2.48 -2.42 -3.93 -2.90 -3.00 -2.93 -4.45 -2.93 -3.03 -2.97 -4.48 -2.39 -2.48 -2.42 -3.93 -1.77 -1.87 -1.81 -3.32 -1.77 -1.87 -1.81 -3.32 -4.77 -4.84 -6.48 -4.80 -4.35 -4.42 -6.06 -4.38 -5.09 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